Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants

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Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants.

Formalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and BCG-FKP compared to MISA-FKP (p < 0.001). Significant increases in splenic lymphocyte recall pro...

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Antileishmanial Activity of Liposomal Clarithromycin against Leishmania Major Promastigotes

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The activity of ozonated olive oil against Leishmania major promastigotes

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Role of imiquimod as adjuvant for vaccination against Leishmania major infection in BALB/c mice

Various adjuvants in combination with different antigens have been utilized as a vaccine candidate against leishmaniasis. However, the search for ideal adjuvants is still pursued due to the inefficiencies of current compounds. In the present study, the effect of imiquimod, as an adjuvant, is studied with soluble Leishmania antigens (SLA) in BALB/c mice. Four groups of mice were immunized with S...

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Partially Induced Protection by a Fraction of Leishmania Major Promastigotes Against Murine Leishmaniasis

Partially purified antigenic fractions of Leishmania major promastigotes were obtained by sodium dodecyl sulfate (SDS-PAGE) and electro-elution. Three isolated protein fractions designated as frac‌tions (Fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kDa) respectively. They were used for immunization of BALB/c mice against Leishmaniasis. The effects of these fractions on immune...

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ژورنال

عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo

سال: 2010

ISSN: 0036-4665

DOI: 10.1590/s0036-46652010000200006